ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Fast ferritin immunoassay on PDMS microchips

Walter Schrott, Marek Nebyla, Lucie Meisterová, and Michal Přibyl

Department of Chemical Engineering, Institute of Chemical Technology, Prague, Technická 5, 166 28 Prague 6, Czech Republic

E-mail: pribylm@vscht.cz

Abstract: A heterogeneous sandwich immunoassay of ferritin on a poly(dimethylsiloxane) microfluidic chip is proposed. An undemanding “prepolymerization technique” based on wet treatment of a phosphor bronze substrate was used for the microchip fabrication. Receptor rabbit antibodies were immobilized via passive sorption directly on microchannel walls. After the incubation of ferritin samples, secondary biotinylated antibodies were introduced. A solution of avidin molecules labeled by fluorescein isothiocyanate was finally added into the microchannels. Lamp-based fluorescence detection of the immunocomplex was then carried out. Dynamic detection range of the method was in the interval from 100 ng mL⁻¹ to 10 μg mL⁻¹.

Keywords: ferritin – immunoassay – microchip – fluorescence – passive sorption – lamp-based detection

Full paper is available at www.springerlink.com.

DOI: 10.2478/s11696-010-0079-6

Chemical Papers 65 (2) 246–250 (2011)