ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Piperine-loaded biogenic stabilized Selenium(0) nanoconjugates for synergistic antimicrobial and neuroprotective activities on peroxide-induced Parkinson-type SH-SY5Y cell

Sujata M. Nikalje, Vijay J. Sawant, Amit R. Supale, Sandeep V. Nipane, and Vikas J. Sawant

Department of Chemistry, Sangli, India

E-mail: VikasSawant@dyp-atu.org

Received: 5 June 2025  Accepted: 27 September 2025

Abstract:

Wet chemical combination of Hibiscus rosa-sinensis flower extract and selenous acid (H₂SeO₃) resulted in biogenic Selenium(0) nanoparticles. The chemical bottom-up approach was used to produce piperine-loaded flavonoid surface-capped Selenium(0) nanoconjugates. These Selenium(0) nanoparticles and piperine-loaded nanoconjugates were characterized using ultraviolet–visible absorption studies, Fourier-transform infrared spectroscopy, and photoluminescence emission spectroscopic techniques to confirm the formation of nanoparticles. The crystallite sizes, packing, and morphologies of Selenium(0) and piperine-loaded Selenium(0) nanoconjugates were determined from X-ray diffraction (XRD) patterns and transmission electron microscopy analysis. The XRD analysis had exhibited the amorphous nature of nanoconjugates with mean size 56 nm. After confirmation of physiochemical properties of piperine-loaded Selenium(0) nanoconjugates, primary in vitro synergistic biocompatibility was studied and compared with free drug piperine over E. coli and Staphylococcus aureus. The neuroprotective effects of nanoconjugates were estimated by in vitro MTT assay and eosin dye staining images of Parkinson neural cell lines with SH-SY5Y model. These nanoconjugates had exhibited synergistic antimicrobial and neuroprotective effects to repair of neuron peroxide damaged cells from cell viability on Parkinson model cells with good biomedical potential.

Graphical abstract

Keywords: Biogenic Selenium; Piperine drug; Nanoconjugates; Neuroprotective

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-025-04421-w

Chemical Papers 80 (1) 681–699 (2026)