ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Fabrication of aptasensor for monitoring cancer biomarkers: detection of lung-specific biomarkers using chemically converted graphene

Xiuxiu Wang, Jiqun Geng, Hui Jiang, Dadeng Gao, Jun Che, Minwei Gu, and Mingjin Xu

Department of Respiratory Medicine, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, Qingdao, China

E-mail: guminwei@163.com

Received: 6 March 2025  Accepted: 21 August 2025

Abstract:

This work was focused on developing a stable, selective, and sensitive electrochemical aptasensor based on 3,4,9,10-perylene tetracarboxylic acid-functionalized chemically converted graphene (PTCA/CCG)-modified electrode for monitoring neuron-specific enolase (NSE), lung-specific biomarkers. The electrochemical aptasensor relied on the purified NSE aptamer immobilized on PTCA/CCG-modified electrode. The PTCA/CCG promoted both the immobilization of the aptamer and the electrochemical signal. The aptasensor exhibited a low limit of detection (0.008 ng/mL), a broad linear range (10^–3 to 10³ ng/mL), remarkable specificity, excellent repeatability and long-term stability, maintaining initial resistance at 96.57% after 30 days. Results exhibited recoveries ranging from 98.00% to 99.00% and relative standard deviation less than 4.08%, demonstrating the acceptable precision and reliability of the fabricated aptasensor and confirming the aptasensor’s performance and efficiency in complex biological fluids and clinical applications.

Keywords: Biomarker; Aptasensor; Graphene; Cancer; Stability

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-025-04382-0

Chemical Papers 79 (12) 9141–9152 (2025)