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Tannins analysis from different medicinal plants extracts using MALDI-TOF and MEKC

Alina Oana Matei, Florentina Gatea, Eugenia Dumitra Teodor, and Gabriel Lucian Radu

aNational Institute for Biological Science, Splaiul Independentei 296, 060031, Bucharest, Romania

 

E-mail: gl_radu@chim.upb.ro

Abstract: The matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF) and micellar electrokinetic chromatography (MEKC) methods were used to identify and quantify five tannins, (+)-catechin, (–)-epigallocatechin, (–)-epigallocatechin gallate, (–)-epicatechin gallate and (–)-epicatechin, from aqueous, ethanolic and acetonic extracts of Calendula officinalis, Hypericum, perforatum, Galium verum and Origanum vulgare. The MALDI-TOF technique was used for screening tannins monomers and oligomers in plant extracts. The sandwich method and matrix 2,5-dihydroxybenzoic acid with a concentration of 10 mg mL–1 in acetonitrile/ultrapure water/trifluoroacetic acid (20 : 80 : 0.1, vol.) were used. The electrophoretic method developed for the separation and quantification of 5 catechins in 15 min exhibited good efficiency and precision, low limits of detection (0.0032–0.0153 μg mL–1) and quantification (0.0096–0.0466 μg mL–1). The correlation coefficients (R2) exceeded 0.9986 and the recovery values ranged between 94.25 % and 102.50 %. The present work provides new information on some of the less studied compounds present in plants frequently used in traditional medicine.

Keywords: tannin – plant extract – MALDI-TOF – MEKC

Full paper is available at www.springerlink.com.

DOI: 10.1515/chempap-2015-0222

 

Chemical Papers 70 (4) 515–522 (2016)

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