ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7
A simple turbidimetric flow injection system for saccharin determination in sweetener products
Camila Bitencourt Mendes, Emiliane Pereira Laignier, Maisa Ribeiro Pereira Lima Brigagão, Pedro Orival Luccas, and César Ricardo Teixeira Tarley
Departamento de Cięncias Exatas, Universidade Federal de Alfenas, Unifal - MG, Alfenas/MG, 37130-000, Brazil
Received: 3 June 2009 Revised: 29 August 2009 Accepted: 10 September 2009
Abstract: A new method for saccharin determination in liquid sweetener products was developed. The method is based on the precipitation reaction of Ag(I) ions with saccharin in aqueous medium (pH 3.0), using a flow injection analysis system with merging zones, the suspension was stabilized with 5 g L−1 Triton X-100. All experimental parameters influencing the flow injection system were optimized by means of chemometric approaches. The linear analytical curve was built from 2.4 g L−1 up to 9.64 g L−1 (r = 0.9968) with a quantification limit of 2.40 g L−1. The precision assessed as relative standard deviation (n = 10) was found to be 1.75 % for the saccharin concentration of 7.20 g L−1. Based on interference studies performed with the substances commonly found in liquid sweeteners, such as sodium cyclamate, methylparaben, sodium aspartame, and benzoic and citric acids, at the analyte to interferent mole ratio of up to 1: 10, no interference with the saccharin determination was observed. The presence of chloride ions interferes with the method, but a preceding liquid-liquid saccharin extraction with ethyl acetate was successfully employed to overcome this drawback. Accuracy of the method in sweetener products was evaluated by a comparison with the HPLC method.
Keywords: saccharin - flow injection system - turbidimetry - factorial design - Doehlert design - sweetener products
Full paper is available at www.springerlink.com.
Chemical Papers 64 (3) 285–293 (2010)