ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7
Synthesis and identification of immunogen medroxyprogesterone acetate residues in edible foods and preparation of the Antisera
C. L. Xu, C. F. Peng, K. Hao, Z. Y. Jin, and S. Q. Yang
School of Food Science and Technology, Southern Yangtze University, Wuxi, 214036, China
Received: 31 March 2005 Revised: 21 July 2005 Accepted: 28 July 2005
Abstract: Medroxyprogesterone acetate (MPA), relative molecular mass is only 344.5 and it has no immunogenecity. The methods of the carbodiimides and the mixed anhydride were both adopted to couple MPA with bovine serum albumin, the carrier protein. The coupling rates of conjugate using the above methods were estimated to be 14 and 20 by ultraviolet spectrophotometry. The coupling was successful according to the analysis of SDS-PAGE illustration. New Zealand white rabbits were immunized with the conjugate the coupling rate of which was 14, and blood was collected after five periods of immunities. Then the titre of antiserum was tested to be 2.6 × 105 by indirect ELISA, which further identified the success of coupling.
Full paper is available at www.springerlink.com.
Chemical Papers 60 (1) 61–64 (2006)