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Separation of Oligogalacturonic Acids by Dextran Gel Chromatography

Ľ. Rexová-Benková

Institute of Chemistry, Slovak Academy of Sciences, Bratislava 9

 

Abstract: From a mixture of oligogalacturonic acids obtained by partial acid hydrolysis or by enzymatic digestion of pectic acid the first five homologues of D-galacturonic acid were isolated by gel chromatography on Sephadex G-25 — fine with 0.05 м phosphate buffer, pH 7 as eluens. When water or 0.01 M phosphate buffer were used as eluens the oligogalacturonic acids were excluded from the column with minimum differences in elution volumes and consequently no separation of the oligogalacturonic acids was achieved; With 0.05 M phosphate buffer Sephadex G-25 — fine acts as molecular sieve where the elution volumes of the oligogalacturonic acids are approximately linear functions of the logarithm of their molecular weights.

Full paper in Portable Document Format: 241a59.pdf

 

Chemical Papers 24 (1) 59–62 (1970)

Thursday, May 13, 2021

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