ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
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Dipeptide Enzymatic Synthesis in a Two-Phase Membrane Reactor

A. Trusek-Holownia and A. Noworyta

Institute of Chemical Engineering, Wroclaw University of Technology, PL-50-373 Wroclaw, Poland

 

E-mail: trusek@iic.pwr.wroc.pl

Received: 19 May 2000

Abstract: The application of a two-phase system in enzymatic synthesis of bitter dipeptide ZAlaPheOMe was studied. Enzymatic conversion was accompanied by the reactant extraction in membrane contactor. Under experimental conditions, reaction kinetics and reactant mass transfer rates were evaluated and the main resistance of process was estimated. The Hatta number was used to the determination of the ratio of diffusion time to the reaction time. It was shown that the process ran in the kinetic region. Owing to a high enzyme activity and stability and the product extraction to organic phase, the substrate conversion degree 0.8 was achieved after 20 h of reaction time.

Full paper in Portable Document Format: 546ba442.pdf

 

Chemical Papers 54 (6b) 442–447 (2000)

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