ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Composition changes of the mixed mobile phase within LC column due to pressure variations A possibility of estimating column packing homogeneity

T. Macko, A. Poťmáková, and D. Berek

Polymer Institute, Centre for Chemical Research, Slovak Academy of Sciences, CS-842 36 Bratislava

Abstract: Sudden changes of pressure within liquid chromatographic (LC) column cause perturbations in the composition of mixed effluent — eigenzones. The extent of this effect depends on the nature of column packing, on the nature and composition of mixed mobile phase, as well as on the relative pressure variation and on the sorbent surface available per volume unit of column, i.e. on the packing density. On the other hand, the size of eigenzones is independent of the eluent flow rate. It is possible to use deliberately generated pressure changes within columns for estimating the homogeneity of their packing beds.

Full paper in Portable Document Format: 432a285.pdf

Chemical Papers 43 (2) 285–296 (1989)