ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Structure—activity relationships for in vitro oxime reactivation of chlorpyrifos-inhibited acetylcholinesterase

K. Kuča, V. Račáková, and D. Jun

Centre of Advanced Studies, University of Defence, 500 01 Hradec Králové, Czech Republic

E-mail: kucakam@pmfhk.cz

Received: 27 July 2006  Revised: 7 March 2007  Accepted: 15 March 2007

Abstract: Organophosphorus pesticides parathion, chlorpyrifos, and malathion inhibit the enzyme acetylcholinesterase (AChE; EC 3.1.1.7) via phosphorylation of its active site. AChE reactivators and anticholinergics are compounds used as antidotes in the case of intoxication by these AChE inhibitors. In this work, chlorpyrifos, a representative member of this pesticide family, was used to inhibit the AChE activity of rat brain. The effect of twenty-one structurally different AChE reactivators was tested in vitro and subsequently, the relationship between their chemical structure and biological activity was outlined.

Keywords: acetylcholinesterase - reactivators - structure—activity relationship - pesticide - organophosphates

Full paper is available at www.springerlink.com.

DOI: 10.2478/s11696-007-0030-7

Chemical Papers 61 (4) 256–261 (2007)