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Citric acid production from raw glycerol by acetate mutants of Yarrowia lipolytica

W. Rymowicz, A. Rywińska, B. Żarowska, and P. Juszczyk

Department of Biotechnology and Food Microbiology, Faculty of Food Science, Agricultural University of Wroclaw, PL-50-375 Wroclaw, Poland



Received: 16 March 2006  Revised: 11 May 2006  Accepted: 4 June 2006

Abstract: Three acetate mutants of the yeast species Yarrowia lipolytica were screened using batch cultivation. The strain Y. lipolytica 1.31 was found to be the most suitable for citric acid production from raw glycerol, a by-product of biodiesel production from rapeseed oil. At the initial concentration of glycerol of 200 g dm−3, the citric acid production of 124.5 g dm−3, yield of 0.62 g g−1, and productivity of 0.88 g dm−3 h−1 were achieved.

Keywords: citric acid - cultivation - glycerol - Yarrowia lipolytica

Full paper is available at

DOI: 10.2478/s11696-006-0071-3


Chemical Papers 60 (5) 391–394 (2006)

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