ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
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New chemiluminometric method for the determination of azithromycin in a continuous flow injection analysis system

Sarah F. Hameed, Inass Hasan Al-Khafaji, and Nagham Shakir Turkey

General Directorate of Education, Ministry of Education, Baghdad, Iraq

Received: 1 January 2024 Accepted: 22 March 2025

Abstract:

A novel analytical method is developed for the determination of azithromycin. The method utilizes continuous flow injection analysis to enhance the chemiluminescence system of luminol, H₂O₂, and Cr(III). The method demonstrated a linear dynamic range of 0.001–100 mmol L^-1 with a high correlation coefficient (r) of 0.9978, and 0.001–150 mmol L^-1 with a correlation coefficient (r) of 0.9769 for the chemiluminescence emission versus azithromycin concentration. The limit of detection (L.O.D.) of the method was found to be 18.725 ng.50 µL⁻¹ based on the stepwise dilution method for the lowest concentration within the linear dynamic range of the calibration graph. The relative standard deviation (R.S.D. %) for n = 6 was less than 1.2% for azithromycin concentrations of 10 mmol L^-1 and 70 mmol L^-1. The developed method was successfully applied for the determination of azithromycin in three pharmaceutical drugs. An analysis of variance (ANOVA) test revealed no significant difference between the reference method and the developed method.

Keywords: Analytical Chemistry; Bioluminescence Imaging; Chemical Diagnostics; Flow Chemistry; Luminescence Spectroscopy; Urinalysis; Azithromycin; Chemiluminescence; Flow injection analysis; Luminol; Cr(III); ISNAG-CL-PA

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-025-04031-6

Chemical Papers 79 (6) 3785–3798 (2025)

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