ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7
Cuttlefish bone powder as an efficient solid-phase extraction sorbent of anti-SARS-CoV-2 drugs in environmental water
Ines Tlili, Rafika Bensghaier, Latifa Latrous El Atrache, and Adel Megriche
Laboratoire de Chimie Minérale Appliquée (LR19ES02), Faculté des Sciences de Tunis, Université de Tunis El Manar, Tunis, Tunisie
Received: 1 April 2022 Accepted: 19 July 2022
Many antiviral drugs were developed to counteract coronavirus disease, 2019 (COVID-19) with severe acute respiratory syndrome. Therefore, the scientific community's efforts have focused on the detection and quantification of antiviral compounds currently being tested for COVID-19 treatment. Cuttlefish bone powder (CFBP) has been used for the first time as solid-phase extraction (SPE) sorbent for the extraction of SARS CoV-2 antiviral drugs (chloroquine, ritonavir and indomethacin) from water samples. An effective and sensitive method was developed by combining SPE and liquid chromatography- UV detection (LC-UV). An experimental design was applied for the optimization of extraction process. Experimental variables were optimized using Doehlert matrix. The developed method included 50 mg of CFBP sorbent, 20 mL of water sample at pH = 9 and 5 mL of ACN/KH2PO4 buffer solution (80:20, v/v) in the elution step. For validation of the method, selectivity, linearity precision, and sensitivity were evaluated. Extraction recovery percentage of all Sars cov-2 antivirals were above 98.2%. The detection and quantification limits were between 0.1 and 0.5 µg L−1 and 0.6 and 2 µg L−1, respectively. The current study suggested that CFBP has the application potential for the enhanced SPE of SARS CoV-2 antiviral drugs from water samples.
Keywords: Green materials; Cuttlefish bone powder; SARS CoV-2 antiviral drugs; Solid-phase extraction; HPLC–UV and chemometrics
Full paper is available at www.springerlink.com.
Chemical Papers 76 (11) 6941–6951 (2022)