ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7
Determination of risperidone at picogram level in human urine by luminol—H2O2 chemiluminescence
X. F. Xie, X. D. Shao, and Z. H. Song
Department of Chemistry, Northwest University, Xi’an, 710069, China
Received: 13 January 2006 Revised: 9 March 2006 Accepted: 20 March 2006
Abstract: A simple flow-injection chemiluminescence method with synergistic enhancement has been investigated for the rapid and sensitive determination of antipsychotic risperidone. The synergistic action was significant in the chemiluminescence system of luminol—hydrogen peroxide with risperidone as an enhancer. The increased chemiluminescence intensity was correlated with risperidone concentration within the range from 10 pg mL−1 to 1.0 ng mL−1 with relative standard deviations lower than 5.0 % and the detection limit of 4 pg mL−1. At a flow rate of 2.0 mL min−1, the flow-injection chemiluminescence method exhibited both a high sensitivity and excellent selectivity giving a throughput of 120 times per hour. The proposed method was successfully applied to determine the risperidone content in human urine without any pretreatment. It was found that the excretive amounts of risperidone reached their maximum after taking 2.0 mg of risperidone for 1 h, with a total excretive ratio of 17.37 % in 8.5 h.
Keywords: chemiluminescence - human urine - risperidone - flow-injection analysis
Full paper is available at www.springerlink.com.
Chemical Papers 60 (4) 288–292 (2006)