ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7

Published monthly
 

Evaluation and identification of antioxidative components of Radix Rhodomyrti by DPPH–UPLC–PDA coupled with UPLC–QTOF-MS/MS

Huihui Yin, Xiaowen Ji, Fei Meng, Wu Zhao, Yuanming Jiang, Zhenhua Qin, Jianhua Sun, and Wei Liu

Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, People’s Republic of China

 

E-mail: weiliu_0771@163.com

Received: 14 August 2020  Accepted: 28 January 2021

Abstract:

Radix Rhodomyrti is the dried root of Rhodomyrtus tomentosa (Aiton) Hassk., which is used as a herb in southeast Asian countries and regions. Its main active ingredients are phenols. To make efficient use of the active components in Radix Rhodomyrti, 2,2-diphenyl-1-picrylhydrazyl radical–ultra-high-performance liquid chromatography–photo diode array (DPPH–UPLC–PAD) analysis and UPLC–quadrupole time-of-flight tandem mass spectrometry (UPLC–QTOF-MS/MS) were applied to evaluate and identify the antioxidant phenolic components in Radix Rhodomyrti. Gallic acid and ellagic acid were identified as the major phenolic components in Radix Rhodomyrti. The levels of gallic acid and ellagic acid in Radix Rhodomyrti were 2.16 ± 0.27 mg/g and 0.17 ± 0.04 mg/g, respectively. Both compounds showed excellent free-radical scavenging activity in vitro. The known antioxidant capacity of ellagic acid is slightly higher than that of gallic acid, which is consistent with the results of the DPPH radical-scavenging assay. The results of this study indicate that the proposed method could be applied for rapid screening and identification of antioxidants from complex natural products.

Keywords: Radix Rhodomyrti; Antioxidant; Gallic acid; Ellagic acid; DPPH–UPLC–PDA

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-021-01544-8

 

Chemical Papers 75 (7) 3273–3281 (2021)

Tuesday, July 27, 2021

IMPACT FACTOR 2020
2.097
SCImago Journal Rank 2020
0.344
SEARCH
Advanced
VOLUMES
XXVIII. International Conference on Coordination and Bioinorganic Chemistry
© 2021 Chemical Papers